Journal: Acta Pharmaceutica Sinica. B
Article Title: Polymer-assisted PD-L1 degradation and targeted photodynamic therapy synergize to suppress immunodeficient tumors
doi: 10.1016/j.apsb.2025.05.022
Figure Lengend Snippet: In vitro immune induction expression testing. (A) CRT in different experimental groups. Scale bar = 50 μm. (B) Columnar analysis depicting CRT induction in different experimental groups. (C) Intracellular ATP release post-administration of treatments. (D) Expression analysis of secreted HMGB-1 protein in cells after treatment administration. (E) In vitro TNF α expression profiles in different treatment groups. (F) In vitro IL-6 expression patterns in different treatment groups. (G) In vitro IFN- γ expression profiles in different treatment groups. (H) Analysis of the in vitro -induced maturation outcomes of DCs. α represents the combination of HPC and HPAP, l represents light and d represents dark. Data are presented as mean ± SD ( n = 3). The P values were calculated by ANOVA with Tukey's test. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001.
Article Snippet: After a 12 h incubation period in standard culture media, the cells were subjected to various treatment conditions, including PBS, HPAP, HPC-dark, HPC-light, (HPC + HPAP)-dark, and (HPC + HPAP)-light groups, for an additional incubation period of 24 h. Subsequent to these treatments, for the intricacies of immune response analysis, culture media supernatants were harvested and assayed for the presence of cytokines TNF α , IL-6, and IFN- γ employing an ELISA protocol detailed by the manufacturer (MultiSciences Biotech Co., Ltd.
Techniques: In Vitro, Expressing
